Flow Cytometry Facility - Available Cell Sorters
The following cell sorters are available for DBM internal and collaborating researchers. In general the cell sorting is performed as a service, conducted and monitored by an operator however it is also possible for researchers to get trained on the FACSAria III cell sorter.
Please contact the flow cytometry facility staff if you would like to do a cell sorting or if you have any other questions regarding the services provided. The potential availability of a cell sorting slot may be checked out by visiting the IRIS reservation website.
Two BD Influx cell sorters with four laser, fifteen parameters and five Lasers twenty-four parameters respectively are available for use. The Influxes are so called “jet-in-air” sorters which makes them slightly faster compared to cuvette based sorters such as the FACSAria. The influxes are therefor in particular well suited for the throughput of larger sample volumes.
Two BD FACSAria cells sorters with with four lasers, seventeen parameters and five lasers, twenty parameters respectively are available for use. As a cuvette based cell sorter, the Arias are in general slightly slower compared to “jet-in-air” sorters such as the influxes. However, the cuvette design makes it more sensitive for detecting low level fluorescence and thus makes it better suited for some multi-colour experiments.