DBM Histology Core Facility

The Histology Core Facility enable researchers to prepare tissue specimens for paraffin embedding, sectioning, cryosectioning and histological stains. It also assists in the setting up and optimizing of histological assays specific for each scientific project.

The facility is equipped with two Leica 1950 cryostats (one also equipped with Cryojane Tape Transfer frozen sectioning system), a Microm HM 340E rotary microtome, a Microm HM 430 Sliding Microtome,  a Medite TPC15 tissue processor for specimen embedding and a new TES Valida embedding station, a Medite CTO20 tissue stainer for hematoxylin and eosin stain, a SlideMate AS Slide Printer and a KOS microwave tissue processor for antigen retrieval and special stains.

The access to the equipment is restricted to trained users.

Please contact Diego Calabrese or Francine Wolf for information and for introduction.

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Leica Cryostat CM1950 1

Clinical cryostat for sectioning frozen OCT embedded tissue
- Quick freezing system
- Safe and easy blade handling
- Automatic and manual sectioning
- UV-C light decontamination system

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Micron HM 340E Microtome

Leica Cryostat CM1950 2


Clinical cryostat for sectioning frozen OCT embedded tissue
- Quick freezing system
- Safe and easy blade handling
- Automatic and manual sectioning
- UV-C light decontamination system
- Tape-transfer system for cryosectioning of delicate tissue

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Micron HM 340E Microtome

Microm HM 340E Microtome

Electronic rotary microtome for formalin-fixed paraffin embedded tissue
- Peltier-cooled specimen clamp
- Zero-orientation head
- Section thickness ranging from 0.5 to 100µm
- Temperature controlled water bath included

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Leica Cryostat CM1950 I (right side)

Continuous Linear Stainer COT 20

Continuous linear automatic stainer for hematoxylin and eosin staining

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KOS microwave tissue processor

Multifunctional microwave tissue processor. Protocols preloaded for: Rapid bone decalcification Special stains Time and temperature controlled antigen retrieval Gross hardening Fixation

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Microm HM 430 Sliding Microtome

Sliding microtome for sectioning FFPE tissues


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TES Valida

Paraffin sample embedding station

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Tissue Processing Center TPC 15 Duo

Closed linear Tissue Processing System with 15 stations for rapid dual sample processing. Parallel processing of two different paraffin embedding programs

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SlideMate AS Slide Printer 

On-demand slide printer with color touch-screen, built-in scanner, two USB ports, one ethernet port, Wi-Fi, bluetooth

Available as add-on equipment for cryostats and microtomes trough the IRIS system

 

 

 

Dr. Diego Calabrese
Department of Biomedicine
University Hospital Basel
Hebelstrasse 20
4031 Basel

Phone: +41 (0)61 328 63 35
diego.calabrese-at-unibas.ch

Francine Wolf
Department of Biomedicine
University Hospital Basel
Hebelstrasse 20
4031 Basel

Phone: +41 (0)61 265 23 87
francine.wolf-at-usb.ch