Before you start: Microscopy pitfalls:
Before you publish: Ethics and data manipulation, from JCB:
SVI Nyquist Calculator:
General introduction to image analysis and automation in microscopy:
The following websites offer comprehensive learning for all aspects of microscopy and are a great resource:
Image J is a powerful open source image processing and analysis program.
There are a lot of plugins for all kind of purposes available.
Fiji is an image processing package.
It can be described as a distribution of ImageJ together with Java, Java 3D and a lot of plugins organized into a coherent menu structure.
IrfanView is a very fast, small, compact and innovative freeware (for non-commercial use) graphic viewer. (For PC only)
It contains a nice batch conversion (with image processing) to change picture formats and sizes. (e.g. tiff to jpg)
Automator is a tool that is already included in the MAC operating system. It can also be used for batch conversion and for much more.
ImageJ tutorials on youtube:
Cellprofiler on youtube:
Introduction to confocal microscopy, Kurt Thorn:
Introduction to fluorescence microscopy, Nico Sturman:
Introduction to live cell, photoactivation, bleaching and super resolution, Jennifer Lippincott-Schwartz:
Introduction to super resolution imaging, Xiaowei Zhuang, Stefan Hell: