Equipment

The facility is equipped with all the necessary devices to perform the basic histology techniques.


Paraffin Sample Processing

 

 

Tissue Processing Centre

Closed linear Tissue Processing System with 15 stations for rapid dual sample processing. Parallel processing of two independent paraffin embedding programs

TES Valida

Paraffin sample embedding station

Microm HM 340E & Microm HM 355S

Electronic rotary microtomes.

Electronic rotary microtome for formalin-fixed paraffin embedded tissue sectioning
- Peltier-cooled specimen clamp
- Zero-orientation head
- Section thickness ranging from 0.5 to 100µm
- Temperature controlled water bath included

Microm HM 430 Sliding Microtome

Manual sliding microtome for formalin-fixed paraffin embedded tissue sectioning

HistoGel warming plate

The HistoGel warming plate is essential to encapsulate and retain even small, friable and viscous specimens during histological processing. This device allows for the correct warming of the Richard-Allan Scientific™ HistoGel™ Specimen Processing Gel.


Frozen Sample Processing

 

 

Leica Cryostat CM1950

Two cryostats for frozen OCT embedded tissue sectioning

- Quick freezing system
- Safe and easy blade handling
- Automatic and manual sectioning
- UV-C light decontamination system
- One also equipped with the tape-transfer system for delicate tissue sectioning and collection


Fresh Tissue Slices

Leica VT1200 S

The vibratome Leica VT1200 S is designed to meet the highest sectioning demands for cutting fresh and fixed tissue.

To achieve sections of the highest quality that retain viable cells on the section surface, the vertical deflection of the blade can be measured by Leica’s Vibrocheck™ measurement device and minimised with the innovative blade holder.


Stains

H&E stain (COT20 linear stainer)

Continuous linear stainer set-up for the hematoxylin and eosin stain.

Hematoxylin and Eosin staining is one of the principal stains in histology and it is the most widely used stain in medical diagnosis.

The current protocol makes use of Papanicolaou's solution 1a Harris' hematoxylin solution and a mixture of Eosin and Erythrosin B.

Tissues stained by this method show:

  • Nuclei in blue/purple
  • Cytoplasm in red
  • Mitochondria in pale pink

Safranin O stain

A manual slide staining station with all the reagents to perform the Safranin O stain is available.

This method is used for the detection of cartilage, mucin, and mast cell granules.

The cartilage and mucin will be stained orange to red, and the nuclei will be stained black. The background is stained bluish green.

 

 

 

 


Microwave Tissue Processor

KOS microwave tissue processor

Multifunctional microwave tissue processor. Protocols preloaded for rapid bone decalcification, special stains, time and temperature controlled antigen retrieval, gross hardening and fixation


Sample Labelling

SlideMate AS & PrintMate AS

The SlideMate AS and the PrintMate AS are a slide printer and a tissue cassette printer respectively, which will avoid mislabelling, and streamline your labelling process by printing slides and cassettes on-demand. The two printers will be connected to a PC and will use the same software for the sample registration and an easy sample tracking.


Slide Mounting

ClearVue Coverslipper

To boost speed and accuracy by automating the coverslipping workload