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Tackling in vivo heart tissue complexity in vitro: a novel culture platform applying multiphysical physiological stimuli paves the way (Marsano Lab)


Clinically relevant three-dimensional (3D) models of human derived cardiomyocytes often lack the complexity of in vivo cardiac tissue organization. Addressing this need, Prof. Anna Marsano and her team report the development and characterization of a medium/high-throughput microscope integrated bioreactor.  In order to mimic the in vivo heart environment, this bioreactor integrates two different kinds of mechanical loading as well as electrical stimulation to engineer reproducible and contractile 3D cardiac tissue derived from neonatal rat or human-induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs). This novel bioreactor could be used as a platform to investigate heart in vitro models of development and pathophysiology as well as for drug testing and the development of patient specific treatments.

This study has recently been published in “iScience (Cell press)”.