Flow Cytometry Facility - Analyzers
The following analyzers are available for independent use after training. To schedule an induction or book the training or consultation, don't hesitate to contact the DBM Flow Team at flowteam-dbm@clutterunibas.ch.
Cytek® Aurora – three at Hebelstrasse and two at Mattenstrasse
The Aurora provides full spectra flow cytometry analysis from 365-829 nm. All three Auroras at DBM are equipped with five spatially separated lasers: 355nm (20mW) / 405nm (100mW) /488nm (50mW) / 561nm (50mW) / 640nm (80mW), three scattering channels (488nm FSC/SSC, 405nm SSC), and 64 fluorescence channels. Acquisition speed of up to 29,000 events/s and measurement of the volume acquired during recording is also possible. Combined loader for 5ml tube, 5ml tube rack and 96 well plate allows automatization of the acquisition process.
The detection over the entire emission spectra allows the simultaneous use of very similar dyes and the detection of very similar fluorescent proteins. It extends the number of markers that can be collected in one sample. The Aurora can resolve rare and dim signals thanks to its low-noise technology. Autofluorescence can be corrected and extracted as a parameter during the measurement, defining multiple cell populations with different autofluorescence and improving the detection of weak fluorescence markers.
BD LSRFortessa – three at Hebelstrasse and one at Mattenstrasse
Five lasers (355nm, 405nm, 488nm, 561nm and 640nm), 18 fluorescence parameters and the fast acquisition make Fortesas analysers of choice for trained DBM researchers using, typically, up to 12-colour multiparameter panels.
BC CytoFLEX– three at Hebelstrasse and two at Mattenstrasse
The facility at Hebelstrasse is provided with three CytoFLEX analysers:
All CytoFLEX S have four lasers (405nm, 488nm, 561nm, 640nm), up to 13 fluorescence colours simultaneously detectable, and both tube and 96-well plate loaders.
At Mattenstrasse we have two CytoFLEX analysers:
CytoFLEX S with four lasers (405nm, 488nm, 561nm 640nm) and CytoFLEX LX with five lasers (355nm, 405nm, 488nm, 561nm 640nm).
The CytoFLEX is controlled by intuitive software (CytExpert®), allowing one to perform a wide range of multicolour experiments with short training.
Amnis ImageStreamX MkII
The ImageStreamX Mk II has three objectives, 20x, 40x and 60x, as well as four lasers, 405nm, 488nm, 560nm and 658nm. It delivers multiple images of every cell in flow, including brightfield, darkfield (SSC), and up to 10 fluorescent markers at an acquisition speed of up to 5,000 cells per second.
Flow cytometry's speed, sensitivity, and phenotyping abilities are combined with the detailed imagery and functional insights of fluorescence microscopy. This unique combination enables a broad range of applications typical for microscopy, quantified on the high number of cells: translocation from the cytoplasm to the nucleus, the study of cell organelles, changes in cell morphology, internalization, co-locatization, cell-to-cell interactions etc.
Detailed analysis of intensity, location, and co-location of probes is achieved using IDEAS® (Image Data Exploration and Analysis Software). IDEAS offers powerful tools for high content, statistically robust analysis of images, standard flow cytometry graphing tools and statistics for hundreds of morphological features in addition to intensity.
CyTOF XT & Hyperion XTi
The CyTOF XT is a next-generation mass cytometer that uses metal isotope-tagged antibodies to profile single cells with unmatched depth and precision. By employing mass spectrometry instead of fluorescence, it avoids spectral overlap, enabling the simultaneous analysis of 50+ parameters from a single sample.
Coupled with the Hyperion XTi Imaging System, these capabilities extend into the spatial context. The Hyperion XTi enables imaging mass cytometry (IMC), allowing the analysis of protein or mRNA expression directly on tissue sections while preserving tissue architecture. Researchers can visualize up to 40 markers simultaneously at subcellular resolution, revealing cellular phenotypes, functional states, and interactions in situ. This powerful combination allows for a truly integrative approach, linking deep cellular profiling with spatial biology to uncover new insights in immunology, oncology, and other complex biological systems.
NanoFCM Analyzer
The nanoFCM Analyzer is a benchtop nanoparticle flow cytometer designed for high-resolution, single-particle characterization of biological and synthetic nanoparticles, including extracellular vesicles (EVs), viruses, liposomes, and inorganic particles. It measures size, concentration, and biochemical properties for particles as small as 7 nm, using ultra-sensitive side-scatter and single-photon counting fluorescence detection.
Its configuration includes 488 nm and 640 nm excitation lasers, two fluorescence detection channels, and side-scatter detection, enabling both label-free measurements and fluorescence-based biochemical profiling. This setup allows precise subpopulation analysis, drug loading assessment, and nucleic acid localization.
Miltenyi Biotec AutoMACS Pro
The AutoMACS Pro is a fully automated bench-top magnetic sorter that can be used to perform sterile bulk sort...
The autoMACS Pro Separator is a bench-top instrument that performs high-speed, gentle cell separation using magnetic activated cell sorting (MACS) technology. It isolates specific cell populations from diverse sources like blood or tissue by utilizing MACS MicroBeads conjugated to antibodies. Users load a magnetically labeled cell suspension, select a separation program on the intuitive touchscreen, and the instrument automatically performs positive or negative selection through MACS columns, providing pure cell fractions for downstream applications such as flow cytometry, cell culture, or omics studies.
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