Flow Cytometry Facility - Analyzers
The following analyzers are available for our researchers. Please contact the flow cytometry facility staff to schedule an introduction or if you have any questions regarding the services provided.
Three identically configured five laser, twenty parameter LSRFortessas (BD Biosciences) are available for trained users. These cytometers offer high sensitivity detection of up to 18 fluorescent labels, at 20K cells per second, paired with easy to use intuitive software. Excitation lasers at 355nm, 405nm, 488nm 561nm and 640nm.
We have one CytoFLEX with the 488nm, 405 and 640nm lasers and up to 11 fluorescence colours plus two CytoFLEX S with 4 lasers (405nm, 488nm, 561nm 640nm) and up to 13 fluorescence colours. The CytoFLEX has an intuitive software and allows for multicolour experiments with high performances alongside a small footprint. Together with the 96 well plate reader this instrument allows for a high through put read out.
Amnis ImageStreamX Mark II
This instrument combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination enables a broad range of applications, such as, translocation from the cytoplasm to the nucleus, differentiation of cells populations by cell morphology, internalization and co-locatization... - the possibility of applications is extremely broad.
The ImageStreamX Mk II has 4 lasers, 405nm, 488nm, 560nm and 658nm and delivers multiple images of every cell in flow, including brightfield, darkfield (SSC), and up to 10 fluorescent markers at up to 5,000 cells per second.
Detailed analysis of intensity, location, and co-location of probes is achieved using IDEAS® (Image Data Exploration and Analysis Software). IDEAS offers powerful tools for high content, statistically robust analysis of images, as well as standard flow cytometry graphing tools and statistics for hundreds of morphological features in addition to intensity.