Cell sorting on the BD FACSAria III, BD FACSDiscover™ S8, and Cytek Spectral CS sorters is provided as a specialist-assisted service. Independent sorting is available on the BD FACSMelody, BD FACS SORP, and Beckman Coulter CytoFLEX SRT after completion of user training.

Please contact the DBM Flow Team at flowteam-dbm@unibas.ch

BD FACSDiscover™ S8 Spectral Cell Sorter - one at Hebelstrasse

The BD FACSDiscover™ S8 is a next-generation cell sorter that unites spectral flow cytometry with high-speed, real-time imaging for powerful, multiparametric cell analysis and sorting.

Equipped with five lasers (349, 405, 488, 561, and 637 nm) and 78 fluorescence channels, the S8 offers unmatched flexibility for experimental design, its SpectralFX Technology captures the full emission spectrum of each fluorochrome and applies advanced spectral unmixing algorithms to accurately distinguish even closely overlapping signals while minimizing interference from autofluorescence. This enables the creation of large and complex multicolor panels with clean, reliable data.

The integrated CellView™ Image Technology acquires up to 10,000 images per second in three fluorescence and two scatter channels, extracting 18 morphological parameters in real time. This unique capability allows researchers to distinguish true doublets from coincident events and sort cells based on protein localization, DNA distribution, or other spatial features.

S8 sorter supports up to 6-way sorting, offers multiple nozzle sizes (85 µm, 100 µm, 130 µm), and enables high-throughput single-cell sorting directly into 96- and 384-well plates

Cytek Aurora CS - One at Hebelstrasse, one at Mattenstrasse 

The Cytek Aurora CS is a state-of-the-art spectral cell sorter that combines 5-laser, 64-channel full-spectrum detection with real-time unmixing to deliver high-resolution analysis of even the most complex cell populations.

Designed for flexibility, it supports up to 6-way sorting, offers multiple nozzle sizes (70 µm, 85 µm, 100 µm, 130 µm) to accommodate diverse cell types, and enables high-throughput single-cell sorting directly into 96-well plates — making it ideal for advanced research in immunology, oncology, and cell biology.

BD FACSAria – two at Hebelstrasse, one at Mattenstrasse

The BD FACSAria III is a versatile, high-performance cell sorter designed for precise and reliable analysis of complex cell populations.

At our Hebelstrasse site, we have two BD FACSAria cell sorters: BD FACSAria SORP with five lasers (355nm, 405nm, 488nm, 561nm, 640nm) and 18 fluorescence parameters, which matches our Fortessa analysers and BD FACSAriaIII with four lasers (405nm, 488nm, 561nm 640nm).

At the Matenstrasse site, we have a four-laser BD FACSAriaIII (405nm, 488nm, 561nm, 640nm).

It delivers high-resolution sorting and supports up to 4-way sorting with 70 µm, 85 µm and 100 µm nozzle sizes to accommodate a variety of cell types. The system also enables high-throughput single-cell sorting directly into 96-well plates and 384-well plates, making it ideal for advanced research in immunology, oncology, and other cell biology applications.

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Beckman Coulter CytoFLEX SRT

The Beckman Coulter CytoFLEX SRT is an advanced benchtop cell sorter designed for high-performance flow cytometry. It offers up to four lasers (405nm, 488nm, 561nm and 640nm) and 15 detection channels, allowing researchers to perform multi-parametric analysis with a high level of sensitivity and resolution. The CytoFLEX SRT features an intuitive interface, flexible configurations, and robust sorting capabilities, making it ideal for a wide range of applications such. Individual use is possible 24/7 after training. 

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BD FACSMelody

This user-friendly instrument is the only self-run sorter in our facility. It has nine fluorescence detectors and does not have a red laser (405nm, 488nm, 561nm). Both small and medium-sized particles can be sorted into tubes or plates. The intuitive and easy-to-learn software FACSChorus™ leads the user step-by-step into a streamlined workflow for setting up a sorting. This system anticipates potential issues and identifies the causes, guaranteeing the quality of the unsupervised sorts. Individual use is possible 24/7 after training.