Inflammatory Bowel Disease . Mucosal Immunology . MacrophageDendritic Cell . T Cell
Mononuclear phagocytes in mucosal immune responses
Inflammatory bowel diseases (IBD), such as Crohn`s disease and ulcerative colitis,are chronic relapsing diseases with increasing incidence and prevalence in the developed world. Innate and adaptive immune responses to constituents of the intestinal microbiota are essential for the development of IBD. Intestinal mononuclear phagocytes are in direct contact with the intestinal microbiota, initiate innate immune responses and shape adaptive immune responses. Phagocytes are located in the lamina propria of the gastrointestinal tract, in cryptopatches and isolated lymph follicles in close proximity to innate lymphoid cells (ILCs). Infection of animals with the mouse pathogen C. rodentium leads to IL-22 production by ILCs,which regulates the expression of REG family proteins required for the defense to infections with enteric pathogens. The depletion of phagocytes decreased IL-22production by ILCs. This means that phagocytes support IL-22 production by ILCs required for host defense in the gut (Manta et al, Mucosal Immunol, 2013).
Mononuclear phagocytes may also initiate adaptive immune responses in the gutas indicated by the close proximity of phagocytes to T cells in the lamina propria of the gastrointestinal tract. Colonic phagocytes sample continuously fluorescent labelled E. coli. To reduce the complex interactions between phagocytes and T cells in presence of the intestinal microflora with vast array pf potential antigens,an antigen-specific colitis model was developed. In this model the challenge of E.coli expressing the antigen ovalbumin induces colitis in immunodeficient animals(RAG animals) reconstituted with antigen-specific T cells. Ex vivo confocal imaging allows the visualization of phagocytes that have sampled E.coli in proximity toT cells. In vitro studies indicated that the phagocytes deliver the antigen to dendritic cells, which migrate to mesenteric lymph nodes. In mesenteric lymph nodes the dendritic cells prime T cells, which home back to the lamina propria. In the lamina propria phagocytes are able to activate the effector T cells (Rossini et al, Mucosal Immunol, 2014).
These findings support the hypothesis that mononuclear phagocytes in the gastro-intestinal tract are of importance for the sampling of constituents of the microbiota, the initiation of innate and adaptive immune responses. Likely, mononuclearphagocytes play a key role in the pathogenesis of IBD.